Transforming growth factor β1, a prototypical autocrine growth factor, exhibits such a remarkable diversity of biological activity that may stimulate or inhibit cellular proliferation and stimulate extracellular matrix formation depending on the
cell
type. We evaluated the effect of TGF-β1 on proliferation of the human prostatic epithelial cell and fibroblast. Epithelial cells were released from tissues by collagen digestion, and primary and subcultured cells were grown in PFMR-4A medium
supplemented with epidermal growth factor, phosphoethanolamine, cholera toxin, selenium, insulin, hydrocortisone, and bovine pituitary extract. Prostate-derived fibroblasts were cultured using RPMI 1640 medium with 10% fetal bovine serum.
Verification
of prostatic epithelial cells was accomplished by indirect immunofluorescence detection of prostate specific antigen, cytokeratin, and prostate-derived fibroblast was confirmed by immunofluorescence detection of vimentin. Proliferation assay was
performed using 3H-thymidine assay. Time and dose dependant inhibitory effects of TGF-β1 on proliferation of prostatic epithelial cells were noted (86±6%, at 0.1ng/ml and 21±3% at 10ng/ml after 96hr exposure), while TGF-±1 at a high
concentration
stimulated the proliferation of prostate-derived fibroblasts (125±11% at 1ng/ml after 48hr exposure, 118±10% at 1ng/ml after 96hr exposure). However in the absence of fetal bovine serum, TGF-β1, at, a physiologic range of 0.01ng/ml showed
inhibitory
effect on proliferation of prostate-derived fibroblasts. These data show that TGF-β1, a growth modulating cytokine, regulates growth of prostatic epithelial cells and prostate-derived fibrblast in the different way.
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